Genetics / -Omics

Transgenic Mouse Facility


Dr. Alexander Awgulewitsch


MUSC - Darby Children's Research Institute



The Transgenic Mouse Core Facility at MUSC uses the pronuclear microinjection technique for production of transgenic animals. Linearized ultra pure DNA is introduced into the pronucleus of a fertilized FVB mouse oocyte. Surviving oocytes are transferred to pseudopregnant females for gestation. Since 2001, the Facility has produced over 575 transgenic mice for investigators at MUSC.  In addition, we can offer pronuclear injection in the C57BL/6 strain and transient expression work. Both services require an additional fee.


Open to all investigators. An investigator wishing to order these services should contact us before making such a request.