Cellular / Tissue

Flow Cytometry and Cell Sorting

Associate Director 

Amanda C. LaRue, PhD
Associate Professor, Pathology & Laboratory Medicine 

Operations Manager 

Adam Soloff, PhD
Research Assistant Professor, Microbiology & Immunology
Phone: 843-792-4794 

E-Mail: soloff@musc.edu

Research Specialist

Gordon Wiegand
Phone: 843-792-8115
E-Mail: wiegandg@musc.edu

Location: 324 Hollings Cancer Center

Research Specialist

Name: Zachary McPherson
Phone: 843-792-8115
E-Mail: mcphersz@musc.edu

Location: 324 Hollings Cancer Center

Description:

The Flow Cytometry & Cell Sorting (FCCS) Core Facility is co-sponsored as a Hollings Cancer Center (HCC) Core Facility and a University Research Resources Facility. Equipped with state-of-the-art instruments and staffed by experts, the goal of the FCCS Shared Resource is to provide comprehensive analytic flow cytometry and high speed cell sorting services in a cost-effective manner to HCC members. The staff of the resource has considerable expertise in high-speed sorting of rare populations of cells including stem/progenitor cells and epitopespecific T cells and is continually expanding the repertoire of available techniques to meet the needs of the innovative research within the HCC.

Services

  • User-Based (Unassisted) Flow Cytometric Analysis: Equipment is available 24/7 for trained investigators. During regular working hours, operators are available to assist with setup, running samples, and trouble-shooting. 
  • Assisted Flow Cytometric Analysis: An operator is available for the FACSCalibur, LSRFortessa and FACSVerse during regular working hours to provide general assistance as well as standard assays including cell death, proliferation, cell cycle, Ca++ flux, etc.
  • Cell Sorting: The MoFlo cell sorter is the instrument of choice for HCC members interested in sorting rare events or side-population cells as a means of isolating tissue stem cells and cancer stem-like cells. With the addition of an Aerosol Management System, the FACSAria IIu cell sorter is used for sorting populations with biohazard considerations. Due to complexity, all cell sorters are operated only by Flow Cytometry and Cell Sorting Unit staff.


Website: 
 

http://hcc.musc.edu/research/resources/cellevaluationandtherapy/flowcytometry/index.htm